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Advanced Center For Cryo-Electron Microscopy Facility

Indian Institute of Science, Bangalore

Overview

The Advanced Center for Cryo-Electron Microscopy Facility (ACCEM-IISc) at IISc Bengaluru is a world class cryo-EM facility in India, which provides access to the researchers & scientists all over the country to implement single particle cryo-EM and cryo-electron tomography (cryo-ET) for high-resolution imaging and structural characterization of biological macromolecules.

IISc has established this facility for biological sciences research purposes.

 

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Research Projects

  1. High quality high-resolution cryo-EM data collection and cryo-EM grid preparation are performed using Talos Arctica at 200kV using K2
    DED. Any kind of biological sample, like purified homogeneous protein samples, DNA-protein complexes, RNA-protein complexes,
    viruses and virus-like particles are imaged using cryo-EM.
  2. A wide molecular weight range of biological samples, from 100-kDa to 100-MDa (large virus particles) complexes are imaged using the
    Talos Arctica cryo-TEM. At this moment, we don’t recommend to image any biological sample, which is less than 100-KDa. It is better to try X-ray crystallography if molecular weight of biological sample
    is less than 100-kDa.

 

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Recent Publications

  • Kumar S, Panda H, Makhdoomi MA, Mishra N, Safdari HA, Chawla H, Aggarwal H, Reddy ES, Lodha R, Kumar Kabra S, Chandele A, Dutta S, Luthra K. An HIV-1 broadly neutralizing antibody from a clade C infected pediatric elite neutralizer potently neutralizes the contemporaneous and autologous evolving viruses. J Virol. 2018 Nov 14. pii: JVI.01495-18. doi: 10.1128/JVI.01495-18.

  • Safdari, HA, Pandey, S, Shukla, AK, Dutta, S. (2018) Illuminating GPCR Signaling by Cryo-EM. Trends Cell Biol, 28, 591-594. (Corresponding Author)

 

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