Cryo-electron microscopy facility at IISc has a state-of-the-art 200 kV Talos-Arctica electron microscope (FEI/Thermo Scientific) equipped with Gatan K2 Summit Direct Electron Detector, automatic data acquisition Latitude S software package and T12 BioTwin Transmission Electron Microscope. Generally, T12 BioTwin Transmission Electron Microscope is used for negative staining sample visualization before proceeding for data collection in cryo-EM. Depending on the sample homogeneity, sample will be imaged using 200 kV Talos-Arctica electron microscope. The facility is equipped with sample preparation units FEI Vitrobot™ Mark IV, GloQube glow discharge system and Q150T ES carbon coater.
We offer one of the best cryo-EM facility to the Indian researchers to prepare their negative staining & cryo-EM sample, high-throughput automatic cryo-EM data acquisition, high speed data transfer, data storage and automatic movie frame alignment. The facility allows the researchers to prepare their sample, data collection, preliminary image analysis and processing.
Our Mission is to provide an open access of cryo-EM facility to all the researchers on the basis of nominal charge basis or collaboration basis. Simultaneously, renowned IISc professors, postdoctoral fellow, EM managers will provide the proper training to the researchers and users about protein purification for cryo-EM study, cryo-sample preparation, data collection and preliminary data processing. One of our major targets is to create a national facility for all the scientific researchers, so everyone could use this facility for their benefit.
At this moment we are not supporting the full phased data processing pipe line for all the users. Data processing facility and supports are only available for collaborative research projects.
This Facility is supported by Department of Biotechnology (DBT) Infrastructure Grant, Govt of India and DST-FIST grant raised by Molecular Biophysics Unit, IISc.